Covalent Conjugation with Dietary Flavonoids Rutin and Isoschaftoside Ameliorates Intestinal Barrier Dysfunction and Inflammation Induced by Peanut Allergy Protein Arah 3 and Enhances its Antioxidant Properties

Abstract

This study aimed to screen flavonoids with high affinities to Arah 3 from 27 candidates by molecular docking, and to evaluate the effects of flavonoid covalent conjugates on intestinal barrier dysfunction and inflammation induced by Arah 3 as well as the antioxidant activities. Computer simulation showed that rutin and isoschaftoside had better affinity and stability with Arah 3. Characterization results showed that protein folding reduced significantly in the covalent conjugates. Intestinal barrier dysfunction induced by Arah 3 was significantly improved after binding with rutin and isoschaftoside, and the isoschaftoside conjugate had a better effect. Co-culture of Caco-2 and RAW264.7 showed that the levels of IL-6, IL-8 and MCP-1 decreased by about 15.61%, 17.94% (11.23%) and 16.17%, respectively, after conjugation with isoschaftoside (rutin). Besides, the antioxidant capacities of the conjugates were significantly improved. This study may supply new insights into the effects of flavonoids on the adverse effects of Arah 3.

Keywords: peanut protein, dietary flavonoids, covalent binding, allergy, Computer simulation